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Screening of Toxoplasma gondii antibodies and Risk factors among Patients in Asabieh City, Libya

Cite this:
Khalif Ali, W., Shubar, H., Ramadan, A., K. Saadawi, W., Annajar, B., R. Alkhanfas, S., & Doro, B. (2019). Screening of Toxoplasma gondii antibodies and Risk factors among Patients in Asabieh City, Libya. Journal of Medical Biomedical and Applied Sciences, 7(6), 255–259. https://doi.org/10.15520/jmbas.v7i6.192
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Abstract

Toxoplasmosis is a zoonotic disease caused by Toxoplasma gondii. Risk factors include
consumption of undercooked meat, raw vegetables, and unfiltered water.
Aim: This study aims to screen antibodies titer in toxoplasmosis positive patients in
Asabieh city in Libya and to screen risk factors associated with infection in this area.
Study design: Data were collected using a cross-sectional design.
Study Duration and Location: Data were collected from patients of different ages
and gendersattending Ali Omar Ascar hospital, Asabieh, during the period of January
2017 to January 2018.
Methodology: A single blood sample was collected from 150 patients and spun at
3000 rpm to obtain serum. The serum samples were analysedto detect anti-Toxoplasma
IgG and IgM antibodies using enzyme linked immunosorbant assay (ELISA, BioChec)
kit according to manufacturer’s instructions. A self-structured questionnaire was used
to obtain information on farm animal’s contact, the process of vegetables washing,
meatcooking, Water resource and raw water consumption.
Results: The total seroprevalence of toxoplasmosis was 78.6%,out of which IgG and
IgM were 68%, and 77.4% respectively. Several risk factors were identified, including
daily contact with farm animals (82.6% were +ve, P = .0.01), unfiltered water (61.3%
were +ve; P = 0.003).
Conclusion: asabieh area showed a high prevalence of toxoplasmosis, and many environmental
risk factors associated with the infection as animal hosts, human lifestyle
were also identified, that could help to reduce the risk of spreading and transmission
of infection among the populations in the future.

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